Synthetic DNA by Randall A. Hughes

Author:Randall A. Hughes
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

10.Remove the gel from the glass plates, and proceed with visualization or post-staining (see Note 3 ).

11.Check the efficiency of ligation (see Note 14 ). Ideally clear bands should be present at the expected size for the full clip (around 83 bp, or larger if additional sequences have been inserted between the half-clips), with bands corresponding to the half-clips being faint or absent.

3.7 DNA Part Preparation

1.DNA parts can be used in the form of linear PCR products, DNA fragments excised from a plasmid, or linearized or circular plasmids which contain the desired part sequence, provided that such plasmids do not contain the antibiotic selection marker to be used for the final construct (see Note 15 ).

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